SAMPLING INSTRUCTIONS FOR HG TYPE TEST
Soil samples for an HG type test should be collected according to guidelines for soil sampling for an SCN egg count. However, at least 40,000 eggs are needed to set-up the HG test. Therefore, a one gallon-sized soil sample should be sent to the laboratory. If an insufficient number of SCN eggs is present in the submitted sample, the SCN population will be increased on a susceptible soybean host in the greenhouse for one month to generate enough eggs for the test. For soil submitted November through April, this step is required to ensure a uniform, meaningful HG test. Soil submitted should be from fields where SCN-resistant soybeans have been grown, the SCN egg counts are increasing, and the field is highly infested. Our HG type test includes the indicator lines Pickett, Peking, PI 88788, PI 90763, and PI 437654. Results are reported as both HG type and race.
CARE OF SOIL SAMPLES
Proper collection and care of samples is extremely important.
Take samples when soil is moist. Put samples in plastic bags, with the label on the outside. Do not let samples dry out!
Nematodes are sensitive to heat. Keep samples in a cool, dark place until they can be mailed. Do not leave samples in the sun or other areas of high temperature.
PACKAGING AND MAILING
- Fill out the sample submission form as completely as possible.
- Label the sample bags with the submitter's name and sample ID designation given on the sample submission form.
- Place sample and submission form in a cardboard mailing box.
- Mail samples to SCN Diagnostics:
1721 East Campus Loop
University of Missouri
Columbia, MO 65211-5315
For any questions, please call 573-884-9118 or send an email to firstname.lastname@example.org.